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KMID : 0620920180500040039
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Experimental & Molecular Medicine
2018 Volume.50 No. 4 p.39 ~ p.39
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Donor-dependent variation of human umbilical cord blood mesenchymal stem cells in response to hypoxic preconditioning and amelioration of limb ischemia
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Kang In-Sung
Lee Byung-Chul
Choi Soon-Won
Lee Jin-Young
Kim Jae-Jun
Kim Bo-Eun
Kim Da-Hyun
Lee Seung-Eun
Shin Na-Ri
Seo Yoo-Jin
Kim Hyung-Sik
Kim Dong-Ik
Kang Kyung-Sun
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Abstract
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With the rapidly growing demand for mesenchymal stem cell (MSC) therapy, numerous strategies using MSCs for different diseases have been studied and reported. Because of their immunosuppressive properties, MSCs are commonly used as an allogeneic treatment. However, for the many donors who could potentially be used, it is important to understand the capacity for therapeutic usage with donor-to-donor heterogeneity. In this study, we aimed to investigate MSCs as a promising therapeutic strategy for critical limb ischemia. We evaluated MSCs from two donors (#55 and #64) and analyzed the capacity for angiogenesis through in vivo and in vitro assays to compare the therapeutic effect between different donors. We emphasized the importance of intra-population heterogeneity of MSCs on therapeutic usage by evaluating the effects of hypoxia on activating cellular angiogenesis in MSCs. The precondition of hypoxia in MSCs is known to enhance therapeutic efficacy. Our study suggests that sensitivity to hypoxic conditions is different between cells originating from different donors, and this difference affects the contribution to angiogenesis. The bioinformatics analysis of different donors under hypoxic culture conditions identified intrinsic variability in gene expression patterns and suggests alternative potential genetic factors ANGPTL4, ADM, SLC2A3, and CDON as guaranteed general indicators for further stem cell therapy.
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KEYWORD
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Mesenchymal stem cells, Peripheral vascular disease
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